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Flow cell sorter(beckman courter )

Posted:2011-12-26 Views: Font:[ Large Middle Small ]


Instrument name: Flow cytometric (Flow cell sorter)
Type: beckman courter (hereinafter referred to as Gallios)


A, instrument characteristics:

Main testing single-celled 10 color more color fluorescent tags within the sample, the main features: (1) the most and detection 10 color fluorescent marker sample (2) can stimulate the dye for: FITC, PE, PI, ECD, PC5, PC5.5, APC, APC-Alexa750, Qdot series dyes, Pacific Blue, Pacific Orange, Alexa405, etc. (3) a single sample storage space and capacity to meet all kinds of stem cells, rare cells subsets and extremely low content (< 0.01% = cells of analysis (4) cells obtain and analysis speed 20000 cells per second, improve the detection efficiency (5) prior to the scattering light high resolution, able to distinguish between smaller cells or particles, such as bacteria, yeast, etc.; (6) with automatic injection system meet efficient testing.

Specific index, performance is as follows:

A laser: 488 nm solid laser, power and mW; 638 nm solid laser, power a 25 mW; 405 nm solid laser, power 40 mW; Laser fixed calibration, do not need to adjust the light path

A fluorescence probe: 10 fluorescence, two scattering light detectors, filter respectively for 488 nm laser 5:488/10 525/40 575/30 620/30 675/20 695/30 755 LP638nm laser 3:660/20 725/20 755 LP405nm laser 2:450/40 550/40

A and earn 10 color fluorescent signal; Automation, manual or offline compensation; The biggest gain speed 20000 cells per second,

A test cell size: 0.5 u m-50 μ m

A single cell storage space and processing capacity: 24 million cells per second

A scattering light sensitivity: effectively distinguish between fixed platelets and noise signal, identify bacteria and 0.5 u m microspheres

A has 32 samples fully automatic injection system and improve the detection efficiency

A fluorescence detection sensitivity: FITC: 112 MESF; PE: 78 MESF; APC: 85 MESF

A signal capture mode: digital signal

A data resolution: 1000000 channel (20 bits)

A instruments have internal temperature control system: security cell activity

A, main testing project


Through the cells ChanBiao at most standard (within 10 color) fluorescent element marker methods, to test the cell surface, the cytoblastema, the variety of protein, the nuclear antigen, DNA and other biological information, common the test items include:


"The lymphocyte subsets detection; Cells within the cells factor detection; Extracellular (serum, plasma, cultivation qing) cell factors of detection. Cell activity detection and apoptosis detection


"Platelet analysis; Nets woven red blood cell analysis; The cell cycle or times body etc DNA analysis; Leukemia, lymphoma immune classification.


"AIDS diagnosis and treatment evaluation; PNH (paroxysmal sleep sex hemoglobinuria) the diagnosis


"HLA-B27 analysis: the diagnosis of ankylosing spondylitis


"Calcium ion flow detection


"Blood stem cells, embryonic stem cell, neural stem cells, etc. Various kinds of stem cell detection

"Cancer drug resistance more testing





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