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Cell Analysis Laboratory

Posted:2011-12-26 Views: Font:[ Large Middle Small ]


Laboratory Introduction

Cells in the enterporise main characteristics and cells form of cell qualitative and quantitative analysis. The tests included the following three aspects:
(1) use of flow cytometric and the cell surface, the cytoplasm and cell of the nuclear one or more antigens, protein and small molecules to fluorescent marker quantitative analysis, and the different characteristics of single cells and fluorescence way to four of the single-celled composition of the road separates.
(2) using laser scanning microscope to inside the cell a variety of fluorescent marker material for 3 d imaging, positioning detection and half quantitative analysis.
(3) using quantitative analysis of image analyzer cells optical geometric parameters and light density parameters. Cell room 5 machines existing flow cytometric (2 station sorting meter, 3 sets analyzer), 3 a laser scanning microscope (including 1 two-photon, 2 sets of single photon) and 2 sets image analyzer, have standard sample preparation room. The main room for users inside cells for opening test service, also bear a graduate student, undergraduate teaching, and undertake research and scientific research cooperation project, not held national technical training course. Cell room 10 existing staff, including Ph.D degree 4, the master's degree 2 people, the subtropical high five people. Published every year SCI relative articles dozens of paper, including the highest impact factor can reach more than 12.0.

Testing Project
1. Flow Cytometry
? lymphocyte subsets detection: including T, B, NK lymphocytes, CD4 + T, CD8 + T, regulatory T, memory T, naive, Th1 / Th2 sexual T cells, Tc1 / Tc2 cell.
? cells factor testing: cells IFN-γ, IL-4, IL-6, IL-10, IL-12, TNF, etc.
? platelet analysis.
? nets woven red blood cell analysis.
? DC cell analysis.
? leukemia, lymphoma immune classification.
? DNA analysis (the cell cycle, times body).
? cell apoptosis analysis.
? hematopoietic stem cell analysis
? cell membrane potential detection.
? cells pH, calcium in the determination.
? cells active oxygen detection.
? phosphorylated proteins detection.
? chromosome analysis.
? sperm number and activity analysis.
? Flow-FISH method to detect telomere length.
? tumor more medicine resistance test.
? cells of the variety of cell factors detection (CBA method), etc.
? tumor cells and resistance related antigen detection, etc.
? single-celled composition of high-speed sorting.
2. Laser Scanning Microscope
? collection high definition of 2-d or 3-d fluorescence images, single or multiple fluorescence image and image transmission light. Show the microstructure and fluorescence samples were positioning, observe and quantitative nucleus, chromosome, mitochondria, the endoplasmic reticulum, gorky's body, tiny tubes, micro wire, membranes, other cytoskeleton system inside the cell and molecular, etc.
? with dynamic tracking or static methods detection of living cells physiological changes and damage, including cell apoptosis, cell fusion, cellular Ca 2 + and H + plasma changes, cells and the mitochondrial membrane potential, ROS, etc. This system is used to track cells of rapid change fluorescent signals. ? and drug testing across a cell membrane tissues or cells into the process and its orientation.
? complete FRAP detection (protein molecules sports, inversion between cells; communications, membrane liquidity) and FRET detection (two kind of protein interactions between the protein molecules, the changes of rsa).
3. Image Analyzer
? in general organization morphology, from macroscopical to microcosmic measurement tissues or cells geometric configuration parameters, such as the thickness of bony trabeculae, aortic lining in the thickness of the film or the blood vessels of the eye, division, cells or the size of the nuclei.
? tumor can be conducted in pathology in tumor cells form analysis, DNA content analysis and cancer of the gene expression immunohistochemical quantitative analysis, etc.
? can also be used in molecular biology, such as PCR amplification product, in situ hybridization, some hybrid, Northern, Southern electrophoresis with light density quantitative.
? image analyzer or a set of fluorescence in situ hybridization system, can simultaneously dual fluorescent marker or three fluorescently labeled image, and can determine the distance and image FISH fluorescence intensity.

Laboratory Personnel
The Lord let: zhang yu (doctor)
Deputy director: after wu male (doctor)
The researchers: YangJianRu, ChenYue, HeJiHua (doctor), YuanLan (doctor), XinShuQin, MaShiLiang, JiaYongRui, official (doctor)
Telephone: 82805034 (flow), 82802437, 82802389 (laser confocal)





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